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Figure 6 | Plant Methods

Figure 6

From: Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings

Figure 6

Movements of nuclei, plastids, and mitochondria. Stacked deconvolution micrographs of root epidermal cells during a 3 h and 40 min long time-lapse observation. (A) Image captured at 2 h and 46 min into the observation period. Nuclei, plastids, and mitochondria appear blue, red, and green, respectively. Triangles indicate the nuclei. Scale bar = 10 μm. (B) Movements of nuclei tracked from 2 h and 27 min to 3 h and 40 min (a total tracking duration of 103 min). Each track is displayed as a line. Line color indicates the time point, corresponding to the time color bar on the right bottom. Solid-line circles indicate areas where the nuclei moved. Dashed-line circles indicate an area where the nucleus stalled. (C) Movements of plastids. Movements are indicated by solid- and dashed-line circles as in (B). (D) Movements of mitochondria. Movements are indicated by solid- and dashed-line circles as in (B). Notice that the tracks of the plastids and mitochondria are similar to that of the nuclei.

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