Localization of nuclei, plastids, and mitochondria in a 3-D model. The stacked image was converted to a 3-D model (blends projection). In this projection, viewing directions and their transparencies were blended to produce a 3-D perspective of the structure. Viewpoints of each image (A to D) are indicated on the bottom. The box indicates the 3-D coordination of the stacked image. The different colors on each wall indicate the different dimensions. Arrows with a letter indicate the viewpoints of each image. A black ellipse on the bottom of the box indicates the position of a nucleus. (A) The blends projection of the stacked image of Figure 4(A). A white rectangle indicates the area enlarged in (B, C, and D). (B) Top view. A nucleus (blue) interacts with plastids (red) and mitochondria (green). The 3-D coordination was not clear in this viewpoint. (C) Side view of the same nucleus. Note that the plastids lay on the nucleus. Also note that the majority of the organelles localize to the cortex of the cells. (D) Bottom view of the same nucleus. Note that the nucleus lays on the mitochondria. Scale bars = 10 μm.