Figure 1From: Optimized cDNA libraries for virus-induced gene silencing (VIGS) using tobacco rattle virusEffect of cDNA insert length on the silencing of PDS in N. benthamiana leaves. (a) Position and length of NbPDS cDNA fragments used for silencing relative to the full-length NbPDS cDNA. All inserts were in the antisense orientation relative to the TRV coat protein. (b) Chlorophyll a levels in the aerial parts of plants infected with TRV-NbPDS constructs. Buffer control plants received infiltration buffer only and empty TRV plants were infected with TRV lacking a silencing insert. Bars represent mean ± SD (n = 3). (c) Photographs of representative plants from TRV infected plants showing the extent of photobleaching.Back to article page