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Table 2 Detailed results from six independent transformation experiments using the improved protocol.

From: High-throughput Agrobacterium-mediated barley transformation

Experiment Construct Embryo inoculation time Number of embryos Independent hygromycin-resistant plants Transformation Efficiencya
A pBract216 DA 75 20 26.7%
  pBract215 DA 50 15 30.0%
   SD 25 5 20.0%
  pBract217 DA 50 16 32.0%
   SD 25 5 20.0%
B pBract216 DA 25 1 4.0%
   SD 75 23 30.7%
  pBract215 DA 25 8 32.0%
   SD 75 22 29.3%
  pBract217 DA 25 7 28.0%
   SD 75 16 21.3%
C pBract216 DA 50 9 18.0%
   SD 75 20 26.7%
  pBract215 DA 75 17 22.7%
   SD 50 9 18.0%
  pBract217 DA 75 24 32.0%
   SD 50 16 32.0%
D pBract216 SD 75 12 16.0%
  pBract215 SD 75 22 29.3%
  pBract217 SD 75 10 13.3%
E pBract216 SD 100 17 17.0%
  pBract215 SD 75 19 25.3%
  pBract217 SD 75 14 18.7%
F pBract216 DA 25 2 8.0%
   SD 100 25 25.0%
  pBract215 DA 50 18 36.0%
   SD 75 22 29.3%
  pBract217 DA 50 13 26.0%
   SD 75 25 33.3%
Total    1750 432 24.7%
  1. DA = day after (embryos inoculated the day after isolation); SD = same day (embryos isolated and inoculated on the same day). aTransformation efficiency is given as the number of independent transformed lines yielding green, hygromycin resistant plants as a percentage of the number of immature embryos treated.