Table 2 Detailed results from six independent transformation experiments using the improved protocol.
From: High-throughput Agrobacterium-mediated barley transformation
Experiment | Construct | Embryo inoculation time | Number of embryos | Independent hygromycin-resistant plants | Transformation Efficiencya |
|---|---|---|---|---|---|
A | pBract216 | DA | 75 | 20 | 26.7% |
pBract215 | DA | 50 | 15 | 30.0% | |
SD | 25 | 5 | 20.0% | ||
pBract217 | DA | 50 | 16 | 32.0% | |
SD | 25 | 5 | 20.0% | ||
B | pBract216 | DA | 25 | 1 | 4.0% |
SD | 75 | 23 | 30.7% | ||
pBract215 | DA | 25 | 8 | 32.0% | |
SD | 75 | 22 | 29.3% | ||
pBract217 | DA | 25 | 7 | 28.0% | |
SD | 75 | 16 | 21.3% | ||
C | pBract216 | DA | 50 | 9 | 18.0% |
SD | 75 | 20 | 26.7% | ||
pBract215 | DA | 75 | 17 | 22.7% | |
SD | 50 | 9 | 18.0% | ||
pBract217 | DA | 75 | 24 | 32.0% | |
SD | 50 | 16 | 32.0% | ||
D | pBract216 | SD | 75 | 12 | 16.0% |
pBract215 | SD | 75 | 22 | 29.3% | |
pBract217 | SD | 75 | 10 | 13.3% | |
E | pBract216 | SD | 100 | 17 | 17.0% |
pBract215 | SD | 75 | 19 | 25.3% | |
pBract217 | SD | 75 | 14 | 18.7% | |
F | pBract216 | DA | 25 | 2 | 8.0% |
SD | 100 | 25 | 25.0% | ||
pBract215 | DA | 50 | 18 | 36.0% | |
SD | 75 | 22 | 29.3% | ||
pBract217 | DA | 50 | 13 | 26.0% | |
SD | 75 | 25 | 33.3% | ||
Total | 1750 | 432 | 24.7% |