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Figure 4 | Plant Methods

Figure 4

From: High-throughput Agrobacterium-mediated barley transformation

Figure 4

Copy numbers of the luciferase gene within transformed plants, determined by quantitative real-time PCR, given as a percentage of the 260 lines analysed. Note that '<1' refers to samples which gave a positive quantitative real-time PCR signal for luciferase and the internal control gene, but the luciferase signal occurred later than for single copy lines (a later signal normally implies less starting template) indicating there may be a problem with gene rearrangements.

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