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Figure 3 | Plant Methods

Figure 3

From: Visualizing the actin cytoskeleton in living plant cells using a photo-convertible mEos::FABD-mTn fluorescent fusion protein

Figure 3

Time lapse images on the xy axes of a region of an onion epidermal cell expressing mEosFP::FABD-mTn taken before photo-conversion and after 60 seconds exposition through a D-filter (UV/Violet; Ex: BP355–425/Dichr: 455/LP 470) using a 0.5 NA 20× lens, demonstrate the progressive enrichment of red fluorescent species over green protein molecules. Accompanying traces for the ROI depicted in panel A confirm the changes observed visually. Whereas a significant jump in red fluorescence can be seen in Panel B already the amount of green fluorescent molecules is still higher, nearly equal fluorescence values appear in panel C and a significant increase is seen in red fluorescence in panels D and E. Size Bar = 25 μm.

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Plant Methods

ISSN: 1746-4811