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Table 4 Summary of SSR primer screening based on laboratory PCR amplification

From: Genome-wide identification of microsatellites in white clover (Trifolium repens L.) using FIASCO and phpSSRMiner

Total primers tested

Perfect SSR primers

Imperfect SSR primers

Primers with amplicons

Monomorphic primers

Polymorphic primers

Polymorphic perfect SSR primers

Polymorphic imperfect SSR primers

191

63

128

176 (92%a)

60 (31%b)

116 (66%b)

47 (75%c)

69 (54%c)

  1. a percentage of designed primers with PCR amplification products
  2. b percentage of primers with amplicons in GA43, SRVR, and six F1 progeny
  3. c percentage of polymorphic primers within the perfect and imperfect groups
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Plant Methods

ISSN: 1746-4811