A simple way to identify non-viable cells within living plant tissue using confocal microscopy

Table 1 Excitation and emission wavelengths (in nm) used.

	excitation maximum	laser line used	emission maximum	emission collected
SYTOX blue	444	458	480	475 to 500
SYTOX green	504	488	523	510 to 560
SYTOX orange	547	568	570	580 to 610
GFP	457	488	504	510 to 545
Propidium iodide	536	488	617	610 to 650
Fluorescein diacetate	494	488	518	505 to 525

The first and third columns show excitation and emission maxima of the fluorescent dyes and proteins used (as indicated by the suppliers). The second and fourth columns show the excitation and emission wavelengths used in our experiments.

ISSN: 1746-4811