Figure 1
From: A simple way to identify non-viable cells within living plant tissue using confocal microscopy
SYTOX dye variants efficiently stain nuclei in fixed plant material. (A) Live embryo stained with SYTOX green. Only a few cells are stained (fluorescence shown in green), which have been wounded during the dissection of the embryo from the seed coat (arrows). (B) Transmission image of the embryo shown in (A). (C) Fixed embryo stained with SYTOX green. (D) CLSM section through the root of a fixed embryo stained with SYTOX green. The stain penetrates into deeper tissue layers. (E) Higher magnification of (C) showing that predominantly the nuclei are stained in the embryonic cells. (F) Fixed embryo stained with SYTOX orange (fluorescence shown in red). (G) Fixed embryo stained with SYTOX blue (fluorescence shown in blue). All images with the exception of (D) are overlay projections of CLSM images. Scalebars: 10 μm in (D) and (E), otherwise 200 μm.