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Figure 3 | Plant Methods

Figure 3

From: A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors

Figure 3

Linearity and sensitivity of qRT-PCR. cDNA derived from root or shoot RNA was mixed in different ratios (as indicated; total amount of cDNA was 1 ng) and used as template to test transcript abundance of three selected genes (Os03g55610, Os08g38220, and Os12g38200) via qRT-PCR. A linear relationship between root (or shoot) cDNA and expression level of the various genes was observed. Symbols in both panels represent the mean ± SD (n = 3).

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