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Table 1 Significantly differentially phosphorylated consensus peptides.

From: Kinome profiling of Arabidopsis using arrays of kinase consensus substrates

Nr.

t-Test lin.

t-Test log

P

Sequence

Kinase

ID substrate protein

12

0.0096

0.0146

â–¼

PSPKYPGPQ

 

SWISS;P05059;CMGA_BOVIN

27

0.0104

0.0168

â–¼

KYLASASTM

CaM kinase II

SWISS;P02687;MBP_BOVIN

50

0.0440

0.0469

â–¼

LSELSRRRI

ds-RNA-dep. Kinase

SWISS;P12962;IF43_YEAST

73

0.0271

0.0215

â–¼

PASPSPQRQ

Cyclin dep. Kinase 5

SWISS;P17599;SYN1_BOVIN

79

0.0384

0.0454

â–¼

LEKKYVRRD

MCSF Recept. (c-fms)

SWISS;P09581;KFMS_MOUSE

102

0.0453

n.s.

â–¼

KISITSRKA

ERA GTPase

SWISS;P06616;ERA_ECOLI

136

0.0023

0.0016

â–¼

PRRDSTEGF

Protein Kinase A

SWISS;P09201;F16P_YEAST

150

0.0441

0.0472

â–²

KKAESPVKE

 

SWISS;P12839;NFM_RAT

152

0.0037

0.0176

â–¼

KTETSQVAP

Rhodopsin Kinase

SWISS;P02699;OPSD_BOVIN

153

0.0211

0.0254

â–¼

KRKVSSAEG

 

SWISS;P02316;HG14_BOVIN

164

0.0228

0.0212

â–¼

PVSPSLVQG

Gprot Receptor Kinase

SWISS;P08172;ACM2_HUMAN

171

0.0058

0.0065

â–²

LDDQYTSSS

 

SWISS;P24604;TEC_MOUSE

175

0.0253

0.0230

â–²

LGGGTFDIS

DnaK (Hsp70)

SWISS;P04475;DNAK_ECOLI

178

0.0005

0.0081

â–¼

KGATSDEED

Casein Kinase 2

SWISS;P06786;TOP2_YEAST

186

0.0418

n.s.

â–¼

LRRPSDQAV

Protein Kinase A

SWISS;P01126;REL_AVIRE

  1. Lysates from 3 independent biological replicates of plants infected with avirulent P. syringae and mock-treated plants are put on PepChips and spot intensities were quantified (see also supplemental data). P-values derived from paired t-Tests performed on original and logarithmic normalized intensities are given; n.s., not significant (p > 0.05). Substrate proteins from which the peptides are derived and -if known- kinases phosphorylating those substrates are indicated. â–² means higher phosphorylation intensities after treatment with avirulent P. syringae; â–¼ means lower phosphorylation.