Table 1 miRNA, primer and probe sequences
From: Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs
miR156 | miRNA sequence | UGACAGAAGAGAGUGAGCAC |
|---|---|---|
Antisense probe | GTGCTCACTCTCTTCTGTCA | |
Sense probe | TGACAGAAGAGAGTGAGCAC | |
RT primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGTGCTC | |
Forward primer | GCGGCGGTGACAGAAGAGAGT | |
UPL RT primer | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCA ACGTGCTC | |
miR156RC | RT primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTGACAG |
Forward primer | GCGGCGTGTGCTCACTCTCT | |
miR159 | miRNA sequence | UUUGGAUUGAAGGGAGCUCUA |
Antisense probe | TAGAGCTCCCTTCAATCCAAA | |
Sense probe | TTTGGATTGAAGGGAGCTCTA | |
RT primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTAGAGC | |
Forward primer | CGGCGGTTTGGATTGAAGGGA | |
UPL RT primer | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCA ACTAGAGC | |
miR159RC | RT primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTTTGGA |
Forward primer | CGGCGGTAGAGCTCCCTTCAA | |
miR167 | miRNA sequence | UGAAGCUGCCAGCAUGAUCUA |
Antisense probe | TAGATCATGCTGGCAGCTTCA | |
Sense probe | TGAAGCTGCCAGCATGATCTA | |
RT primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTAGATC | |
Forward primer | TCGCGTGAAGCTGCCAGCAT | |
UPL RT primer | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCA ACTAGATC | |
miR167RC | RT primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTGAAGC |
Forward primer | GGCGGTAGATCATGCTGGCA | |
miR171 | miRNA sequence | UGAUUGAGCCGCGCCAAUAUC |
Antisense probe | GATATTGGCGCGGCTCAATCA | |
RT primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGATATT | |
Forward primer | TTCCTTGATTGAGCCGCGCC | |
UPL RT primer | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCA ACGATATT | |
miR166 | miRNA sequence | UCGGACCAGGCUUCAUUCCCC |
RT primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGGGGAA | |
Forward primer | TCGCTTCGGACCAGGCTTCA | |
UPL RT primer | GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCA ACGGGGAA | |
Universal | Reverse primer | GTGCAGGGTCCGAGGT |
CmRBS | Forward primer | ATGGCTTCCATCGTCTCATCCGCC |
Reverse primer | TTGTCGAAGCCAATGACTCTGATGAA | |
CmPP16 | Forward primer | GTGGTAAAGGACTTCAAGCCCACGACC |
Reverse primer | ATGGGTTTGAAGAAGCCAAGCCACTTA |