Three minimum tile paths from bacterial artificial chromosome libraries of the soybean (Glycine max cv. 'Forrest'): tools for structural and functional genomics

Table 1 Progress in the soybean physical map (builds 2 to 4).

	Build 2	Build 3	Build 4	Build 4 repeats	Footnotes
BAC clones in FPC database	81,024	78,001	78,001
BACs used in contig assembly	75,568	76,749	72,942		1, 2
Number of singletons	5,884	3,702	27,810
Marker anchored singletons	0	0	120
Clones in contigs (fold genome)	69,684	73,047	45,130
Fold genome in contigs	8.7	9.1	5.6
Number of contigs	5,597	2,905	2,854	646
Anchoring Markers	0	385	404
Anchored Contigs	0	781	742	181
Q Contigs	n/a	1040	0
Contigs contain:
> 25 clones	220	921	477	268	3
10 – 25 clones	3,038	920	1,458	433	4
3 – 9 clones	1,845	850	820	0
2 clones	385	216	99	0
Unique bands within contigs	396,843	345,457	258,240	54,560	5
Length of the contigs (Mb)	16,676	14,516	1.037	0.258

¹ Adjusted for 1252 "NoFp" 0 band clones only (includes all clones with > = 1 band).
² Adjusted for all 0–4 band clones, contamination-suspect clones and most high band number (>65) clones.
³ All repeat contigs with greater than 25 clones are represented in contig #'s 9000 and greater.
⁴ Includes 265 repeat contigs that have 15–19 clones (contig #'s 8000 – 8264) and 168 contigs that have 20–24 clones in contig #'s 9000 and greater.
⁵ Based on 4.0 kbp per unique band, for 2,208 contigs containing ~68 unique bands in 15 clones, 264 duplicated region contigs containing ~68 unique bands in 30 clones 15,840 unique bands and 406 highly repeated region contigs containing ~68 unique bands in 60 clones, 48,720 unique bands.
⁶ Based on 4.0 kbp per unique band.

ISSN: 1746-4811