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Table 2 Primer design for differential display.

From: Recent developments in primer design for DNA polymorphism and mRNA profiling in higher plants

Traditional differential display system[16]
Forward
5'-AAGCTTXXXXXXX-3'
The XXXXXXX was designed to target the mRNA sequences with a good coverage of mRNA species in the sample.
Reverse
5'-AAGCTTTTTTTTTTTTTA-3'
5'-AAGCTTTTTTTTTTTTTA-3'
5'-AAGCTTTTTTTTTTTTTG-3'
Annealing control primer system[14]
Forward
5'-GTCTACCAGGCATTCGCTTCATIIIIIXXXXXXXXXX-3'
The XXXXXXXXXX was designed to target the mRNA sequences with a good coverage of mRNA species in the sample.
Reverse
5'-CTGTGAATGCTGCGACTACGATIIIIITTTTTTTTTTTTTTT-3'
Multiplex differential display system[15]
Forward
5'-CTTNNXXXXXXXX-3' (N = A, C, G, or T)
The XXXXXXXX was designed to target the mRNA sequences with a good coverage of mRNA species in the sample.
Reverse
5'-AAGCTTTTTTTTTTTTTC-3'
5'-AAGCTTTTTTTTTTTTTG-3'
5'-AAGCTTTTTTTTTTTTTA-3'