Table 2 Primer design for differential display.
From: Recent developments in primer design for DNA polymorphism and mRNA profiling in higher plants
Traditional differential display system[16] |
|---|
Forward |
5'-AAGCTTXXXXXXX-3' The XXXXXXX was designed to target the mRNA sequences with a good coverage of mRNA species in the sample. |
Reverse |
5'-AAGCTTTTTTTTTTTTTA-3' |
5'-AAGCTTTTTTTTTTTTTA-3' |
5'-AAGCTTTTTTTTTTTTTG-3' |
Annealing control primer system[14] |
Forward |
5'-GTCTACCAGGCATTCGCTTCATIIIIIXXXXXXXXXX-3' The XXXXXXXXXX was designed to target the mRNA sequences with a good coverage of mRNA species in the sample. |
Reverse |
5'-CTGTGAATGCTGCGACTACGATIIIIITTTTTTTTTTTTTTT-3' |
Multiplex differential display system[15] |
Forward |
5'-CTTNNXXXXXXXX-3' (N = A, C, G, or T) The XXXXXXXX was designed to target the mRNA sequences with a good coverage of mRNA species in the sample. |
Reverse |
5'-AAGCTTTTTTTTTTTTTC-3' |
5'-AAGCTTTTTTTTTTTTTG-3' |
5'-AAGCTTTTTTTTTTTTTA-3' |