Table 2 Q-PCR experimental details. A list of primer sequences, PCR product sizes and optimal acquisition temperatures for control genes and genes of interest used for the production and normalization of Q-PCR data.
From: Isolation of plant transcription factors using a modified yeast one-hybrid system
Gene | Forward Primer | Reverse Primer | PCR product size (bp) | Acquisition Temperature (°C) |
|---|---|---|---|---|
Ta GAPdH | TTCAACATCATTCCAAGCAGCA | CGTAACCCAAAATGCCCTTG | 220 | 79 |
Ta CyP | CAAGCCGCTGCACTACAAGG | AGGGGACGGTGCAGATGAA | 227 | 83 |
Ta eF-1 alpha | CAGATTGGCAACGGCTACG | CGGACAGCAAAACGACCAAG | 227 | 80 |
Ta HDZipI-1 | CCAGATGGAGCACCACCACG | CACGCTCATTGCTGACCATG | 198 | 83 |
Ta HDZipI-2 | AGAGAGCGAGAGACTCGGTG | AAGAGCAGGGGCGGAAATGG | 200 | 78 |
Ta HDZipII -1 | AGACACCGGAGCAACGTACG | ACGAAGGACTAAACTTTCTG | 180 | 83 |
Ta DREB2 | GCGTACAACACCTTGATTTCC | AAACTCAACTCACATCTAAGC | 182 | 75 |