Figure 1From: A novel system for gene silencing using siRNAs in rice leaf and stem-derived protoplastsRice leaf protoplasts transformed with varying concentrations of a plasmid containing GFP. Protoplasts were prepared from 2-week-old kitaake plants and transformed as described (Methods) with p35S-GFP. Images were taken at 40× magnification under either bright field (top) or a GFP specific filter (bottom). Pictures are representative of two independent experiments for each concentration of plasmid DNA.Back to article page