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Figure 5 | Plant Methods

Figure 5

From: The visible touch: in planta visualization of protein-protein interactions by fluorophore-based methods

Figure 5

Principle of the BiFC assay. The scheme depicts the principle of the BiFC assay, exemplified by a split YFP fluorophore. Proteins A and B are fused to N- and C-terminal fragments of YFP, respectively. In the absence of an interaction between A and B, the fluorophore halves remain non-functional. Following interaction between A and B, a functional fluorophore is reconstituted which exhibits emission of fluorescence upon excitation with an appropriate wavelength.

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Plant Methods

ISSN: 1746-4811