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Figure 2 | Plant Methods

Figure 2

From: The visible touch: in planta visualization of protein-protein interactions by fluorophore-based methods

Figure 2

Detection of protein-protein interactions via FRET. FRET between cyan fluorescent protein (CFP) as a donor fused to protein A and yellow fluorescent protein (YFP) fused as an acceptor to protein B. Under favorable spatial and angular conditions, interaction between A and B causes a decrease in the intensity of donor (CFP) fluorescence concomitant with an increase in acceptor (YFP) fluorescence. CFP and YFP are depicted as cyan and yellow ribbon models fused to putative interacting proteins A and B, respectively.

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Plant Methods

ISSN: 1746-4811