FLS2 -dependency of ROS production in response to live Pto DC3000 and Pto hrcC-cells. Time-course of ROS production in response to Pto DC3000 (A; closed symbols) or Pto hrcC- (B; open sympols) in Col-0 (square) and fls2 null mutant (triangle) leaf disc halves (n = 24/treatment). C: Comparison of peak ROS production between Pto DC3000 or Pto hrcC- in Col-0 and fls2 shown at the point of maximum ROS production (35 minutes post-elicitation from A and B). To allow for direct comparisons, all ROS experiments shown in (A, B and C) were performed in the same 96-well plate at the same time. Values are mean ± SE, means with different letters denote a significance difference while similar letters denote no significance (Two tailed student’s t-test, P < 0.0001). For all experiments, bacterial solutions were used at OD600 = 0.1. Experiments were repeated more than 3 times with similar results. RLU, Relative Light Units.