Figure 4

RbohD and BAK1 -dependency of ROS production in response to live Pto DC3000 and Pto hrcC^-cells. A and B: Time-course of ROS production in response to Pto DC3000 (A; closed symbols) or Pto hrcC^- (B; open sympols) in Col-0 (square) and rbohD null mutant (triangle) leaf disc halves (n = 24/treatment). C: Comparison of peak ROS production between Pto DC3000 or Pto hrcC^- in Col-0 and rbohD shown at the point of maximum ROS production (35 minutes post-elicitation from A and B). D and E: Time-course of ROS production in response to Pto DC3000 (D; closed symbols) or Pto hrcC^- (E; open sympols) in Col-0 (square) and bak1-4 null mutant (triangle) leaf disc halves (n = 24/treatment). F: Comparison of peak ROS production between Pto DC3000 or Pto hrcC^- in Col-0 and bak1-4 shown at the point of maximum ROS production (35 minutes post-elicitation from D and E). To allow for direct comparisons, all ROS experiments shown in (A, B and C) and (D, E and F) were performed in the same 96-well plate at the same time. Values are mean ± SE, means with different letters denote a significance difference while similar letters denote no significance (Two tailed student’s t-test, P < 0.0001). For all experiments, bacterial solutions were used at OD₆₀₀ = 0.1. Experiments were repeated more than 3 times with similar results. RLU, Relative Light Units.