Transient ROS production in response to live Pto DC3000 and Pto hrcC-in wildtype Arabidopsis Col-0. A: Time-course of ROS production in response to Pto DC3000 (n = 48/treatment). B: Time-course of ROS production in response to Pto hrcC- (n = 48/treatment). C: ROS production induced by Pto DC3000 (black bar) and Pto hrcC—(white bar) shown at peak ROS production (35 minutes post-elicitation) and as total Relative Light Units (RLUs) over 80 minutes following elicitation with indicated bacteria (n = 48/treatment). D: Specificity of ROS production based on presence of Arabidopsis tissue and Pto DC3000 cells (n = 12/treatment). E: Specificity of ROS production based on presence of Arabidopsis tissue and Pto hrcC- cells (n = 12/treatment). To allow direct comparisons, ROS experiments in (A, B and C), in D or in E were performed in the same 96-well plate at the same time. Values are mean ± SE, means with different letters denote a significance difference while similar letters denote no significance (Two tailed student’s t-test, P < 0.0001). For all experiments, bacterial solutions were used at OD600 = 0.1. Experiments were repeated more than 3 times with similar results. RLU, Relative Light Units; ns, no significance; DC, Pto DC3000; hrcC, Pto hrcC-; Lum, Luminol; HRP, Horseradish Peroxidase.