Table 6 Comparison of different BR analytical methods
Pretreatment method | Separation/detection | Analyte | LOD | Amount of plant tissues | Analysis time |
|---|---|---|---|---|---|
LLE-MSPE-derivatization[9] | LC-FLD | 24-epiBL | 0.12 ng | 50 g | More than 3 hours |
SPE-ultrafiltration-SPE-derivatization[7] | Online trapping-UPLC-MS/MS | 28-epihomoBL | 0.2 pg | 400 mg | 7 hours |
MCX SPE-MAX SPE-derivatization[32] | UPLC-MS/MS | BL, CS, teasterone (TE), typhasterol (TY) | 1.5-3.9 pg | 1 g | 1 day |
On-line two-dimensional microscale SPE-on column derivatization-HPLC-MS/MS[30] | On-line-HPLC-MS/MS | 24-epiBL, 24-epiCS, 6-deoxo-24-epiCS,TE, TY | 1.4-6.6 pg | 225 mg | 40 minutes |
MSPE coupled with ISD (this work) | UPLC-MS/MS | 28-norBL, 28-norCS, 28-homoBL, BL, CS | 0.1-0.3 pg | 100 mg | 1 hour |