LEAF-E: a tool to analyze grass leaf growth using function fitting

Table 3 Comparison of leaf elongation in four Brachypodium inbred lines

Growth parameter			Bd3-1 (mean ± SE)⁽⁺⁾	Bd21 (mean ± SE)⁽⁺⁾	Bd21-3 (mean ± SE)⁽⁺⁾	Bd2-3 (mean ± SE)⁽⁺⁾
	Lm	(mm)	94^a ± 3	91^a ± 1	113^b ± 3	113^b ± 2
	LERmax	(mm°C^-1d^-1)	1.44^ab ± 0.05	1.29^a ± 0.03	1.52^b ± 0.05	1.51^b ± 0.03
Thermal time points	t20%	(°Cd)	162 ^a ± 3	163^a ± 2	148^b ± 3	166^a ± 2
	t20	(°Cd)	164^a ± 3	166^a ± 2	145^b ± 3	163^a ± 2
	t50%	(°Cd)	189^a ± 2	193^a ± 2	178^b ± 3	197^a ± 2
	tm	(°Cd)	202^a ± 2	206^a ± 2	192^b ± 3	211^a ± 2
	t90%	(°Cd)	217^ab ± 1	222^bc ± 2	210^a ± 4	229^c ± 2
	te	(°Cd)	230.8^ab ± 0.8	238^bc ± 2	226^a ± 4	245^c ± 2
Leaf elongation durations	LED(20-e)	(°Cd)	67^a ± 3	72^a ± 1	81^b ± 2	82^b ± 2
	LED(20%-90%)	(°Cd)	69^a ± 2	75^ab ± 1	78^b ± 1	79^b ± 1
	LED(20%-e)	(°Cd)	55^a ± 2	59^ab ± 1	62^b ± 1	63^b ± 1

Bd3-1 (n = 7), Bd21 (n = 10) plants, Bd21-3 (n = 7) plants, Bd2-3 (n = 10). Lm: final leaf length; LERmax: maximal leaf elongation rate; t20%, t50%, t90%, te: time points at which the leaf reaches 20%, 50%, 90% and 100% of the final leaf length, respectively; tm: time point at which the leaf reaches LERmax; LEDs: leaf elongation durations between above stated thermal time points.
+Statistical significance indicated with distinct letters based on ANOVA and Scheffé Post hoc test (p < 0.05) between lines Bd3-1 (n = 7), Bd21 (n = 10) plants, Bd21-3 (n = 7) plants, Bd2-3 (n = 10), applied base temperature for thermal time calculation = 11°C, Mean of overall R² values = 0.9993 (0.9871-0.9993).

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