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Table 1 Effect of GA20ox1 overexpression on maize leaf elongation

From: LEAF-E: a tool to analyze grass leaf growth using function fitting

Growth parameter

AtGA20ox1 OE (mean ± SE)

Control (mean ± SE)

Difference in mean(+)

 

Lm

(mm)

743 ± 13

535 ± 6

38.9%

***

LERmax

(mm°C-1d-1)

6.2 ± 0.2

4.6 ± 0.1

34.2%

***

Thermal time points

t100

(°Cd)

107 ± 2

108 ± 4

-1.7%

NS

t20%

(°Cd)

121 ± 3

111 ± 4

8.7%

NS

t50%

(°Cd)

165 ± 3

153 ± 5

7.6%

*

tm

(°Cd)

180 ± 4

167 ± 5

7.7%

*

t90%

(°Cd)

217 ± 4

203 ± 5

6.7%

*

te

(°Cd)

246 ± 5

231 ± 5

6.3%

*

Leaf elongation durations

LED(100-e)

(°Cd)

139 ± 4

123 ± 2

13.3%

**

LED(20%-90%)

(°Cd)

96 ± 2

92 ± 2

4.2%

NS

LED(20%-e)

(°Cd)

125 ± 30

120 ± 2

4.0%

NS

  1. A segregating population produced by backcrossing (BC) a transgenic plant overexpressing the Arabidopsis thaliana GIBBERELLIC ACID 20 OXIDASE1 (GA20ox1) gene to the wild-type line B104 was analysed for leaf growth. The results are based on the analysis of eleven transgenic and nine non-transgenic BC1 plants. Lm: final leaf length; LERmax: maximal leaf elongation rate; t20%, t50%, t90%, te: time points at which the leaf reaches 20%, 50%, 90% and 100% of the final leaf length, respectively; t100: time point at which the leaf reaches 100 mm; tm: time point at which the leaf reaches LERmax; LEDs: leaf elongation durations between above stated thermal time points.
  2. +Statistical significance based on student t-test of non-transgenic plants (n = 9) vs GA20ox1 overexpression (n = 11), *p < 0.05, ** p < 0.01, ***p < 0.001, NS non-significant. Applied base temperature for thermal time calculation = 10°C, Mean of overall R2 values = 0.9983 (0.9970-0.9991).
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Plant Methods

ISSN: 1746-4811