|
Growth parameter
|
AtGA20ox1 OE (mean ± SE)
|
Control (mean ± SE)
|
Difference in mean(+)
|
|---|
| |
Lm
|
(mm)
|
743 ± 13
|
535 ± 6
|
38.9%
|
***
|
|
LERmax
|
(mm°C-1d-1)
|
6.2 ± 0.2
|
4.6 ± 0.1
|
34.2%
|
***
|
|
Thermal time points
|
t100
|
(°Cd)
|
107 ± 2
|
108 ± 4
|
-1.7%
|
NS
|
|
t20%
|
(°Cd)
|
121 ± 3
|
111 ± 4
|
8.7%
|
NS
|
|
t50%
|
(°Cd)
|
165 ± 3
|
153 ± 5
|
7.6%
|
*
|
|
tm
|
(°Cd)
|
180 ± 4
|
167 ± 5
|
7.7%
|
*
|
|
t90%
|
(°Cd)
|
217 ± 4
|
203 ± 5
|
6.7%
|
*
|
|
te
|
(°Cd)
|
246 ± 5
|
231 ± 5
|
6.3%
|
*
|
|
Leaf elongation durations
|
LED(100-e)
|
(°Cd)
|
139 ± 4
|
123 ± 2
|
13.3%
|
**
|
|
LED(20%-90%)
|
(°Cd)
|
96 ± 2
|
92 ± 2
|
4.2%
|
NS
|
|
LED(20%-e)
|
(°Cd)
|
125 ± 30
|
120 ± 2
|
4.0%
|
NS
|
- A segregating population produced by backcrossing (BC) a transgenic plant overexpressing the Arabidopsis thaliana GIBBERELLIC ACID 20 OXIDASE1 (GA20ox1) gene to the wild-type line B104 was analysed for leaf growth. The results are based on the analysis of eleven transgenic and nine non-transgenic BC1 plants. Lm: final leaf length; LERmax: maximal leaf elongation rate; t20%, t50%, t90%, te: time points at which the leaf reaches 20%, 50%, 90% and 100% of the final leaf length, respectively; t100: time point at which the leaf reaches 100 mm; tm: time point at which the leaf reaches LERmax; LEDs: leaf elongation durations between above stated thermal time points.
- +Statistical significance based on student t-test of non-transgenic plants (n = 9) vs GA20ox1 overexpression (n = 11), *p < 0.05, ** p < 0.01, ***p < 0.001, NS non-significant. Applied base temperature for thermal time calculation = 10°C, Mean of overall R2 values = 0.9983 (0.9970-0.9991).
