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Table 1 Effect of GA20ox1 overexpression on maize leaf elongation

From: LEAF-E: a tool to analyze grass leaf growth using function fitting

Growth parameter AtGA20ox1 OE (mean ± SE) Control (mean ± SE) Difference in mean(+)
  Lm (mm) 743 ± 13 535 ± 6 38.9% ***
LERmax (mm°C-1d-1) 6.2 ± 0.2 4.6 ± 0.1 34.2% ***
Thermal time points t100 (°Cd) 107 ± 2 108 ± 4 -1.7% NS
t20% (°Cd) 121 ± 3 111 ± 4 8.7% NS
t50% (°Cd) 165 ± 3 153 ± 5 7.6% *
tm (°Cd) 180 ± 4 167 ± 5 7.7% *
t90% (°Cd) 217 ± 4 203 ± 5 6.7% *
te (°Cd) 246 ± 5 231 ± 5 6.3% *
Leaf elongation durations LED(100-e) (°Cd) 139 ± 4 123 ± 2 13.3% **
LED(20%-90%) (°Cd) 96 ± 2 92 ± 2 4.2% NS
LED(20%-e) (°Cd) 125 ± 30 120 ± 2 4.0% NS
  1. A segregating population produced by backcrossing (BC) a transgenic plant overexpressing the Arabidopsis thaliana GIBBERELLIC ACID 20 OXIDASE1 (GA20ox1) gene to the wild-type line B104 was analysed for leaf growth. The results are based on the analysis of eleven transgenic and nine non-transgenic BC1 plants. Lm: final leaf length; LERmax: maximal leaf elongation rate; t20%, t50%, t90%, te: time points at which the leaf reaches 20%, 50%, 90% and 100% of the final leaf length, respectively; t100: time point at which the leaf reaches 100 mm; tm: time point at which the leaf reaches LERmax; LEDs: leaf elongation durations between above stated thermal time points.
  2. +Statistical significance based on student t-test of non-transgenic plants (n = 9) vs GA20ox1 overexpression (n = 11), *p < 0.05, ** p < 0.01, ***p < 0.001, NS non-significant. Applied base temperature for thermal time calculation = 10°C, Mean of overall R2 values = 0.9983 (0.9970-0.9991).