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Figure 10 | Plant Methods

Figure 10

From: Bacterially produced Pt-GFP as ratiometric dual-excitation sensor for in planta mapping of leaf apoplastic pH in intact Avena sativa and Vicia faba

Figure 10

Near neutral leaf apoplast in Avena sativa requires Pt -GFP for detecting alkalizing effects. Leaf apoplastic pH response of Avena sativa as provoked by the addition of 25 mM Cl− via L-cysteinium chloride into the nutrient solution. Time point of chloride addition as indicated by the arrow. pH, as quantified at the adaxial leaf face is plotted over time. Pt-GFP, grey curve; Oregon Green, black curve. Plants were cultivated with 15 mM nitrate in the nutrient solution given as Ca(NO3)2. Leaf apoplastic pH was averaged (n = 6 ROIs per ratio image and time point; mean ± SE of ROIs). Representative kinetics of eight equivalent recordings of plants gained from independent experiments (n = biological replicates).

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Plant Methods

ISSN: 1746-4811