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Table 3 RT reaction solution

From: Protocol: a fast and simple in situ PCR method for localising gene expression in plant tissue

Component Stock conc. Final conc./amount 1× (μL, in-tube) 1× (μL, on-slide)
cDNA synthesis buffer (kit) 4 20
DTT (kit) 0.1 M 5 mM 1 5
RNaseOUT (kit) 40 U μL−1 40 U 1 1
Thermoscript RT (kit) 15 U μL−1 15 U 1 1