Table 3 RT reaction solution
From: Protocol: a fast and simple in situ PCR method for localising gene expression in plant tissue
Component | Stock conc. | Final conc./amount | 1× (μL, in-tube) | 1× (μL, on-slide) |
|---|---|---|---|---|
cDNA synthesis buffer (kit) | 5× | 1× | 4 | 20 |
DTT (kit) | 0.1Â M | 5Â mM | 1 | 5 |
RNaseOUT (kit) | 40 U μL−1 | 40 U | 1 | 1 |
Thermoscript RT (kit) | 15 U μL−1 | 15 U | 1 | 1 |