Metabolite profiling of wheat (Triticum aestivum L.) phloem exudate

Table 2 The mean and standard error of the difference and Fold change for metabolites, profiled using GC-MS, that significantly increased ( p <0.05) in the phloem from 9–11 DAA (n = 15) to 18–20 DAA (n = 16)

Metabolite	Transformation	Levene’s test for equality of variances sig.	t-test for equality of means sig. (2-tailed)	Mean difference	Std. error difference	Fold change
Citric acid 4TMS	None	0.512	0.000	0.23250	0.05283	1.8
Fructose_MX1	None	0.002^un	0.000	0.27792	0.05953	1.8
Fumarate 2TMS	None	0.000^un	0.004	0.00942	0.00289	1.6
Gluconic acid-1,5-lactone 4TMS	None	0.066	0.002	0.34643	0.09990	1.7
Glucose MX1	None	0.008^un	0.002	0.63124	0.17488	1.7
Glycine 3TMS	None	0.026^un	0.000	0.17284	0.03384	2.2
Hexadecanoate 1TMS	SQRT	0.004^un	0.017	0.27188	0.10404	1.9
Methionine 1TMS	None	0.337	0.024	0.00554	0.00233	1.3
Octadecanoate 1TMS	SQRT	0.028^un	0.021	0.21070	0.08527	1.8
Phenylalanine 2TMS	None	0.654	0.018	1.07470	0.42754	1.5
Putrescine 4TMS	None	0.697	0.000	0.82294	0.13397	1.9
Quinic acid 5TMS	SQRT	0.010^un	0.007	0.32413	0.10951	2.5
Shikimic acid 4TMS	SQRT	0.060	0.000	0.23331	0.05785	2.9
Succinate 2TMS	None	0.000^un	0.010	0.05240	0.01802	2.3
Tyrosine 3TMS	None	0.180	0.000	1.09938	0.24564	1.8
UN04_15.56_185	None	0.000^un	0.019	0.10668	0.04183	1.8
UN10_19.08_217	None	0.106	0.007	0.44254	0.15290	1.4
UN16_25.71_339	None	0.016^un	0.015	0.00806	0.00302	1.7
UN17_27.24_375	None	0.003^un	0.000	0.01570	0.00379	1.8
UN18_28.91_437	None	0.011^un	0.001	0.01317	0.00358	1.6
UN20_32.34_503	InvCBRT	0.020^un	0.049	-0.62079	0.29828	1.8

Also shown are the p values for Levene’s test of equality of variance, where values are less than 0.05 (equal variances not assumed = ^un), equal sample variances were not assumed when calculating t-test. (x TMS = Trimethylsilyl derivative where x = the number of TMS groups; y MX = methoxyamine derivatised product where y = 1 or 2).

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