Metabolite profiling of wheat (Triticum aestivum L.) phloem exudate

Table 1 The mean and standard error of the difference and Fold change for metabolites, profiled using GC-MS, that significantly decreased ( p <0.05) in the phloem from 9–11 DAA (n = 15) to 18–20 DAA (n = 16)

Metabolite	Transformation	Levene’s test for equality of variances sig.	t-test for equality of means sig. (2-tailed)	Mean difference	Std. error difference	Fold change
3-amino-piperidin-2-one 2TMS	SQRT	0.227	0.000	-0.34838	0.07402	-3.4
Alanine 2TMS	SQRT	0.000^un	0.009	-0.23127	0.08092	-1.9
Arginine 3TMS	Ln	0.286	0.000	-0.84617	0.20727	-2.3
Glutamate 3TMS	None	0.003^un	0.005	-1.11392	0.35445	-2.3
Glutamine 3TMS	CBRT	0.296	0.001	-0.34581	0.09220	-2.6
Histidine 3TMS	Ln	0.994	0.032	-0.62065	0.27622	-1.9
Homoserine 3TMS	None	0.613	0.000	-0.08141	0.01851	-1.9
Lysine 4TMS	None	0.180	0.006	-0.57860	0.19365	-1.4
Ornithine 3TMS	None	0.001^un	0.007	-0.17965	0.05689	-4.6
Pyroglutamate 2TMS	None	0.444	0.018	-3.21496	1.28551	-1.3
Serine 3TMS	None	0.073	0.000	-4.85198	1.16680	-1.6
Trehalose 8TMS	Ln	0.952	0.000	-1.25509	0.19795	-3.5
UN01_10.61_158	CBRT	0.360	0.000	-0.07041	0.01492	-2.3
UN07_17.62_275	Ln	0.177	0.002	-0.68564	0.20462	-2.0
UN08_17.96_360	CBRT	0.673	0.001	-0.19065	0.05177	-3.4
UN09_18.15_275	CBRT	0.363	0.003	-0.08408	0.02541	-2.0
UN11_19.48_299	CBRT	0.003^un	0.003	-0.08195	0.02451	-2.0
UN26_14.48_229	Ln	0.034^un	0.001	-0.61709	0.15206	-1.9

Also shown are the p values for Levene’s test of equality of variance, where values are less than 0.05 (equal variances not assumed = ^un), equal sample variances were not assumed when calculating t-test. (x TMS = Trimethylsilyl derivative where x = the number of TMS groups; y MX = methoxyamine derivatised product where y = 1 or 2).

ISSN: 1746-4811