Table 1 Reporter proteins and fluorescence analysis conditions evaluated
Reporter | Laser scanner imaging | Plate reader FI quantification | Species | Structure | Ref. | ||
|---|---|---|---|---|---|---|---|
Laser (nm) | Em (nm) | Ex (nm) | Em (nm) | ||||
mTagBFP2 | n.a.1 | n.a. | 400/9 | 455/20 | Entacmaea quadricolor | Monomer | [31] |
mTFP1 | 457 | 526SP | 450/9 | 480/20 | Clavularia sp. | Monomer | [32] |
mGFP5 | 488 | 526SP | 485/9 | 535/20 | Aequorea victoria | Weak dimer | [29] |
sGFP(S65T) | 488 | 526SP | 485/9 | 535/20 | Aequorea victoria | Weak dimer | [30] |
mNeonGreen | n.t.2 | n.t. | 500/9 | 530/20 | Branchiostoma lanceolatum | Monomer | [33] |
mPapaya1 | 532 | 555/20 | 520/9 | 550/20 | Zoanthus sp. | Monomer | [34] |
TagRFP-T | 532 | 580/30 | 560/9 | 595/20 | Entacmaea quadricolor | Monomer | [35] |