Skip to main content
Figure 9 | Plant Methods

Figure 9

From: AGROBEST: an efficient Agrobacterium-mediated transient expression method for versatile gene function analyses in Arabidopsis seedlings

Figure 9

Transient expression of MYB75 increases anthocyanin accumulation. Four-day-old Arabidopsis efr-1 seedlings were infected with Agrobacterium C58C1(pTiB6S3ΔT)H carrying a vector (pCAMBIA1390), 35S::MYB75, 2X35S::MYB75, super::MYB75, or super::gusA-intron by the AGROBEST method. At 3 dpi, co-cultivation medium was replaced with MS medium containing 100 μM Timemtin for additional incubation for 3 days. qRT-PCR of relative expression of MYB75 (A) and CHS (B) with representative data shown with mean ± SD from 3 technical repeats. Similar results were obtained from three independent experiments. Zeiss inverted microscopy of anthocyanin accumulation in seedlings (upper panels) and cotyledons (lower panels) and quantification (C). Data for anthocyanin content are mean ± SD from 4 repeats (2 biological repeats from each of 2 independent experiments, 20–30 seedlings for each biological repeat), Values significantly different from that obtained with vector are denoted (**P < 0.01, by Student’s t test).

Back to article page

Plant Methods

ISSN: 1746-4811