Figure 4

AGROBEST enables high transient expression levels in Col-0. Four-day-old Arabidopsis seedlings were infected with Agrobacterium strain C58C1(pTiB6S3ΔT)^H carrying pBISN1 (A and C) or 35S::LUC2 (B), and transient expression activity was determined at 3 dpi. (A) Transient GUS expression efficiency of Col-0 and efr-1 seedlings by AGROBEST, FAST and Marion et al. methods. Data for quantitative GUS activity are mean ± SD of 3 biological replicates. Values significantly different from those obtained with Col-0 by AGROBEST are denoted (*P < 0.05 by Student’s t test). (B) Transient luciferase expression efficiency of Col-0 and efr-1 seedlings by AGROBEST, FAST and Marion et al. methods. Seedlings infected by C58ΔvirB2 carrying 35S::LUC2 were used as a background control and those without Agrobacterium infection are indicated as MOCK. Luciferase activity of Col-0 obtained by AGROBEST was set to 100% and that of others is relative to activity of Col-0 by AGROBEST. Data are mean ± SD of 3 biological replicates. Values significantly different from those obtained with Col-0 by AGROBEST are denoted (**P < 0.01, by Student’s t test). (C) Transient GUS expression efficiency of Col-0, AvrPto transgenic line, and efr-1 by AGROBEST. For dexamethasone (DEX) treatment, 3-d-old seedlings were treated with 10 μM DEX for 1 day and the following 3 days infected by the AGROBEST method. Quantitative GUS activity from DEX-induced Col-0 seedlings by AGROBEST was set to 100% and that of others is relative to activity of DEX-induced Col-0 seedlings with AGROBEST. Data are mean ± SD GUS activity from 4 repeats (2 biological repeats from each of 2 independent experiments). Values significantly different from that obtained with Col-0 are denoted (**P < 0.01 by Student’s t test). Seedlings grown in the same co-cultivation medium without Agrobacterium infection are indicated (MOCK).