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Figure 1 | Plant Methods

Figure 1

From: AGROBEST: an efficient Agrobacterium-mediated transient expression method for versatile gene function analyses in Arabidopsis seedlings

Figure 1

Transient transformation assays in different Arabidopsis ecotype/genotypes. Four-day-old Arabidopsis seedlings were infected with Agrobacterium strain C58C1(pTiB6S3ΔT)H carrying pBISN1, which was pre-incubated in AB-MES (pH5.5) supplemented with 200 μM acetosyringone (AS) to induce vir gene expression. Seedlings were co-cultivated with pre-induced A. tumefaciens cells with final OD600 = 0.02 in the MS medium (1/2 MS, 0.5% sucrose (w/v), pH 5.5) containing 50 μM AS and determined for transient GUS expression levels by overnight GUS staining (A) and quantitative GUS activity (B) at 3 dpi. The GUS activity obtained from Col-0 seedlings was set to 100% and that of Ws, efr-1, and fls2 is relative to that of Col-0. Data are mean ± SD GUS activity from two biological replicates. Similar results were obtained from at least two independent experiments. Values significantly different from that obtained with Col-0 are denoted (*P = 0.058 by Student’s t test).

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Plant Methods

ISSN: 1746-4811