Figure 2
From: Protocol: a beginner’s guide to the analysis of RNA-directed DNA methylation in plants
Quantitative measurement of 24nt siRNAs and scaffold RNAs in Arabidopsis. [A] Quantification of individual 24nt siRNAs by TaqMan small RNA assays. The levels of siRNAs were normalized by using snoR101 as internal control. [B] Quantification of Pol V-dependent scaffold RNAs by gene/scaffold RNA-specific RT-qPCR. Actin2 was used as internal control. Error bars indicate SD, n = 3 technical replicates. All experiments were performed with at least two biological repeats. Representative images are shown.