Figure 2
From: Suitable transfection methods for single particle tracing in plant suspension cells
Transfection of MBs via the standard PEG protocol leads to unspecific conglomerates within the cell. Representative images of protoplasts transfected with a MB labeled with an Atto550 fluorophore targeting exon junction 6 (Ej6) mRNA of RS2Z33. Arrowheads label foci of PEG-MB conglomerates. Arrows depict vacuolar membranes which are also fluorescently labeled by PEG-MBs. Note the difference between the strongly (1st, 3rd and 4th row) and poorly transfected cells (2nd row). On the left side are the transmission images with visible nucleus (marked yellow). V - vacuole; N - nucleus.