Figure 1
From: Suitable transfection methods for single particle tracing in plant suspension cells
The scheme depicts the protein and gene structure of RS2Z33 and its transcripts. A) The Molecular Beacon was designed to bind to exon junction 6 of RS2Z33; i.e. all splice variants of RS2Z33 are targeted. Red – intronic sequences; Black – exonic sequences; white – untranslated regions; RRM – RNA recognition motif; ZnK – Zink knuckle; SR – Serine/Arginine rich domain; SP – Serine/Proline-rich domain; alt 3’-ss – alternative 3’ splice site; IR – intron retention; I2 – intron 2; MB – Molecular Beacon; EJ6 – exon junction 6; F – fluorophore; Q – quencher. B) Illustration of the work principle of MB.