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Figure 1 | Plant Methods

Figure 1

From: Combining metal oxide affinity chromatography (MOAC) and selective mass spectrometry for robust identification of in vivo protein phosphorylation sites

Figure 1

Proposed strategy for robust identification of serine/threonine phosphorylation in plants. In case of low stoichiometry as for the in vivo situation enrichment of phosphoproteins is necessary. To cope with this a novel enrichment procedure called metaloxide affinity chromatography (MOAC) is used in the strategy [18]. The whole approach is applicable to identify phosphorylation sites out of complex samples. The library of in vivo sites may be used for screening of protein phosphorylation dynamics using triple-quadruple mass spectrometry [6].

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