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Table 1 Comparison of predicted and observed microsatellite allele frequency. The number of alleles each locus was predicted to generate as described in Figure 1 was compared to the actual alleles observed when screened over 672 genotypes of wild, uncultivated cocoa. Predictions were based on the height and number of peaks reported from the pooled samples and took account of the extra DNA amplified from the pDrive vector when predicting the size of the DNA fragments.

From: High throughput, high resolution selection of polymorphic microsatellite loci for multiplex analysis

  PREDICTED OBSERVED
Locus min max mode alleles min max mode alleles
mTcCIR080 97 103 101 4 97 105 99 4
mTcCIR131 198 212 212 9 185 214 210 12
mTcCIR155 264 274 272 5 265 275 267 5
mTcCIR190 156 172 162 9 148 174 161 12
mTcCIR065 230 250 240 6 231 255 237 11
mTcCIR066 280 310 287 8 280 308 284 9
mTcCIR069 185 205 202 11 175 206 202 13
mTcCIR088 182 197 189 7 180 200 187 8
mTcCIR092 277 286 282 3 269 284 279 6
mTcCIR103 90 116 112 6 88 128 110 16
mTcCIR113 130 150 142 8 124 153 133 15
mTcCIR158 210 220 212 4 205 227 212 7
mTcCIR172 125 135 126 9 115 140 124 14
mTcCIR195 335 351 348 5 319 349 349 10
mTcCIR203 210 220 216 5 212 218 216 5
mTcCIR266 170 200 177 9 165 206 200 13