Table 1 Comparison of predicted and observed microsatellite allele frequency. The number of alleles each locus was predicted to generate as described in Figure 1 was compared to the actual alleles observed when screened over 672 genotypes of wild, uncultivated cocoa. Predictions were based on the height and number of peaks reported from the pooled samples and took account of the extra DNA amplified from the pDrive vector when predicting the size of the DNA fragments.
From: High throughput, high resolution selection of polymorphic microsatellite loci for multiplex analysis
| Â | PREDICTED | OBSERVED | ||||||
|---|---|---|---|---|---|---|---|---|
Locus | min | max | mode | alleles | min | max | mode | alleles |
mTcCIR080 | 97 | 103 | 101 | 4 | 97 | 105 | 99 | 4 |
mTcCIR131 | 198 | 212 | 212 | 9 | 185 | 214 | 210 | 12 |
mTcCIR155 | 264 | 274 | 272 | 5 | 265 | 275 | 267 | 5 |
mTcCIR190 | 156 | 172 | 162 | 9 | 148 | 174 | 161 | 12 |
mTcCIR065 | 230 | 250 | 240 | 6 | 231 | 255 | 237 | 11 |
mTcCIR066 | 280 | 310 | 287 | 8 | 280 | 308 | 284 | 9 |
mTcCIR069 | 185 | 205 | 202 | 11 | 175 | 206 | 202 | 13 |
mTcCIR088 | 182 | 197 | 189 | 7 | 180 | 200 | 187 | 8 |
mTcCIR092 | 277 | 286 | 282 | 3 | 269 | 284 | 279 | 6 |
mTcCIR103 | 90 | 116 | 112 | 6 | 88 | 128 | 110 | 16 |
mTcCIR113 | 130 | 150 | 142 | 8 | 124 | 153 | 133 | 15 |
mTcCIR158 | 210 | 220 | 212 | 4 | 205 | 227 | 212 | 7 |
mTcCIR172 | 125 | 135 | 126 | 9 | 115 | 140 | 124 | 14 |
mTcCIR195 | 335 | 351 | 348 | 5 | 319 | 349 | 349 | 10 |
mTcCIR203 | 210 | 220 | 216 | 5 | 212 | 218 | 216 | 5 |
mTcCIR266 | 170 | 200 | 177 | 9 | 165 | 206 | 200 | 13 |