Table 1 Detergents successfully employed for the solubilization of protein complexes prior to BN-PAGE.

Dodecylmaltoside

Mammalian

1.1%

[1]

Mitochondria

Plant Mitochondria

1.5%

[103]

Chloroplasts

2.0%

[75]

Yeast Peroxisomes

0.5% and 1.0%

[81]

Digitonin

Mammalian

4 g/g protein

[104]

Mitochondria

Plant Mitochondria

5.0 g/g protein

[6]

Chloroplasts

1.5 g/g protein

[41]

Yeast Microsomes

1.0%

[80]

Yeast Peroxisomes

1.0%

[81]

Triton X-100

Mammalian

1.0, 1.4 and 2.4%

[105]

Mitochondria

Plant Mitochondria

0.5%

[6]

Yeast Peroxisomes

0.2% and 1.0%

[81]

Whole cellular lysates of different human cell lines

0.1%

[3]

Brij 96

Whole cellular lysates of different human cell lines

0.5%

[3]

Octylglucoside

Tobacco microsomes

40 mM

[2]

Saponin

Whole cellular lysates of different human cell lines

1.0%

[3]