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Table 1 Detergents successfully employed for the solubilization of protein complexes prior to BN-PAGE.

From: Blue-native PAGE in plants: a tool in analysis of protein-protein interactions

Detergent Sample Detergent concentration Reference
Dodecylmaltoside Mammalian 1.1% [1]
  Mitochondria   
  Plant Mitochondria 1.5% [103]
  Chloroplasts 2.0% [75]
  Yeast Peroxisomes 0.5% and 1.0% [81]
Digitonin Mammalian 4 g/g protein [104]
  Mitochondria   
  Plant Mitochondria 5.0 g/g protein [6]
  Chloroplasts 1.5 g/g protein [41]
  Yeast Microsomes 1.0% [80]
  Yeast Peroxisomes 1.0% [81]
Triton X-100 Mammalian 1.0, 1.4 and 2.4% [105]
  Mitochondria   
  Plant Mitochondria 0.5% [6]
  Yeast Peroxisomes 0.2% and 1.0% [81]
  Whole cellular lysates of different human cell lines 0.1% [3]
Brij 96 Whole cellular lysates of different human cell lines 0.5% [3]
Octylglucoside Tobacco microsomes 40 mM [2]
Saponin Whole cellular lysates of different human cell lines 1.0% [3]