Figure 2From: Reproducible hairy root transformation and spot-inoculation methods to study root symbioses of peaIllustrations depicting the main steps for the production of composite pea plants. A. Plants grown for 10 days in vermiculite (7 days in dark followed by 3 in light). B. Fibrgro® cubes saturated with A. rhizogenes. C. A shoot of a 10 day-old plant cut above node 2 and inserted into Fibrgro® cube. D. A wilted pea plant about to receive water for recovery. E. Plants placed in growth tray under a plastic dome. F. High humidity is provided by plastic lid with closed ventilation holes and metal tray with water. G. A root (arrow) protruding through a Fibrgro® cube 10 days after A. rhizogenes infection. H. Callus-forming plants, the regenerated roots of which have been excised, placed in an upright position in vermiculite before being inoculated with R. leguminosarum 3 days later.Back to article page