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  1. Methodology

    Reference genes for normalization of qPCR assays in sugarcane plants under water deficit

    Sugarcane (Saccharum spp.) is the main raw material for sugar and ethanol production. Among the abiotic stress, drought is the main one that negatively impact sugarcane yield. Although gene expression analysis th...

    Larissa Mara de Andrade, Michael dos Santos Brito, Rafael Fávero Peixoto Junior, Paulo Eduardo Ribeiro Marchiori, Paula Macedo Nóbile, Alexandre Palma Boer Martins, Rafael Vasconcelos Ribeiro and Silvana Creste

    Plant Methods 2017 13:28

    Published on: 17 April 2017

  2. Methodology

    Protoplast isolation prior to flow cytometry reveals clear patterns of endoreduplication in potato tubers, related species, and some starchy root crops

    Endoreduplication, the process of DNA replication in the absence of cell division, is associated with specialized cellular function and increased cell size. Genes controlling endoreduplication in tomato fruit ...

    F. Parker E. Laimbeer, Sarah H. Holt, Melissa Makris, Michael Alan Hardigan, C. Robin Buell and Richard E. Veilleux

    Plant Methods 2017 13:27

    Published on: 14 April 2017

  3. Methodology

    Ex vitro hairy root induction in detached peanut leaves for plant–nematode interaction studies

    Peanut (Arachis hypogaea) production is largely affected by a variety of abiotic and biotic stresses, including the root-knot nematode (RKN) Meloidogyne arenaria that causes yield losses worldwide. Transcriptome ...

    Larissa Arrais Guimaraes, Bruna Medeiros Pereira, Ana Claudia Guerra Araujo, Patricia Messenberg Guimaraes and Ana Cristina Miranda Brasileiro

    Plant Methods 2017 13:25

    Published on: 11 April 2017

  4. Methodology

    A real-time phenotyping framework using machine learning for plant stress severity rating in soybean

    Phenotyping is a critical component of plant research. Accurate and precise trait collection, when integrated with genetic tools, can greatly accelerate the rate of genetic gain in crop improvement. However, e...

    Hsiang Sing Naik, Jiaoping Zhang, Alec Lofquist, Teshale Assefa, Soumik Sarkar, David Ackerman, Arti Singh, Asheesh K. Singh and Baskar Ganapathysubramanian

    Plant Methods 2017 13:23

    Published on: 8 April 2017

  5. Methodology

    A robust protocol for efficient generation, and genomic characterization of insertional mutants of Chlamydomonas reinhardtii

    Random insertional mutagenesis of Chlamydomonas reinhardtii using drug resistance cassettes has contributed to the generation of tens of thousands of transformants in dozens of labs around the world. In many inst...

    Steve V. Pollock, Bratati Mukherjee, Joanna Bajsa-Hirschel, Marylou C. Machingura, Ananya Mukherjee, Arthur R. Grossman and James V. Moroney

    Plant Methods 2017 13:22

    Published on: 3 April 2017

  6. Methodology

    TIPS: a system for automated image-based phenotyping of maize tassels

    The maize male inflorescence (tassel) produces pollen necessary for reproduction and commercial grain production of maize. The size of the tassel has been linked to factors affecting grain yield, so understand...

    Joseph L. Gage, Nathan D. Miller, Edgar P. Spalding, Shawn M. Kaeppler and Natalia de Leon

    Plant Methods 2017 13:21

    Published on: 31 March 2017

  7. Methodology

    Growth curve registration for evaluating salinity tolerance in barley

    Smarthouses capable of non-destructive, high-throughput plant phenotyping collect large amounts of data that can be used to understand plant growth and productivity in extreme environments. The challenge is to...

    Rui Meng, Stephanie Saade, Sebastian Kurtek, Bettina Berger, Chris Brien, Klaus Pillen, Mark Tester and Ying Sun

    Plant Methods 2017 13:18

    Published on: 23 March 2017

  8. Methodology

    An image analysis pipeline for automated classification of imaging light conditions and for quantification of wheat canopy cover time series in field phenotyping

    Robust segmentation of canopy cover (CC) from large amounts of images taken under different illumination/light conditions in the field is essential for high throughput field phenotyping (HTFP). We attempted to...

    Kang Yu, Norbert Kirchgessner, Christoph Grieder, Achim Walter and Andreas Hund

    Plant Methods 2017 13:15

    Published on: 21 March 2017

  9. Methodology

    The combination of gas-phase fluorophore technology and automation to enable high-throughput analysis of plant respiration

    Mitochondrial respiration in the dark (R dark) is a critical plant physiological process, and hence a reliable, efficient and high-throughput method of measuring variation in rates of R

    Andrew P. Scafaro, A. Clarissa A. Negrini, Brendan O’Leary, F. Azzahra Ahmad Rashid, Lucy Hayes, Yuzhen Fan, You Zhang, Vincent Chochois, Murray R. Badger, A. Harvey Millar and Owen K. Atkin

    Plant Methods 2017 13:16

    Published on: 21 March 2017

  10. Methodology

    Development of an efficient glucosinolate extraction method

    Glucosinolates, anionic sulfur rich secondary metabolites, have been extensively studied because of their occurrence in the agriculturally important brassicaceae and their impact on human and animal health. Th...

    T. Doheny-Adams, K. Redeker, V. Kittipol, I. Bancroft and S. E. Hartley

    Plant Methods 2017 13:17

    Published on: 21 March 2017

  11. Methodology

    The Tree Drought Emission MONitor (Tree DEMON), an innovative system for assessing biogenic volatile organic compounds emission from plants

    Biogenic volatile organic compounds (BVOC) emitted by plants play an important role for ecological and physiological processes, for example as response to stressors. These emitted compounds are involved in che...

    Marvin Lüpke, Rainer Steinbrecher, Michael Leuchner and Annette Menzel

    Plant Methods 2017 13:14

    Published on: 20 March 2017

  12. Methodology

    rosettR: protocol and software for seedling area and growth analysis

    Growth is an important parameter to consider when studying the impact of treatments or mutations on plant physiology. Leaf area and growth rates can be estimated efficiently from images of plants, but the expe...

    Filipa Tomé, Karel Jansseune, Bernadette Saey, Jack Grundy, Korneel Vandenbroucke, Matthew A. Hannah and Henning Redestig

    Plant Methods 2017 13:13

    Published on: 15 March 2017

  13. Methodology

    The Microphenotron: a robotic miniaturized plant phenotyping platform with diverse applications in chemical biology

    Chemical genetics provides a powerful alternative to conventional genetics for understanding gene function. However, its application to plants has been limited by the lack of a technology that allows detailed ...

    Thomas Burrell, Susan Fozard, Geoff H. Holroyd, Andrew P. French, Michael P. Pound, Christopher J. Bigley, C. James Taylor and Brian G. Forde

    Plant Methods 2017 13:10

    Published on: 1 March 2017

  14. Research

    A high-throughput stereo-imaging system for quantifying rape leaf traits during the seedling stage

    The fitness of the rape leaf is closely related to its biomass and photosynthesis. The study of leaf traits is significant for improving rape leaf production and optimizing crop management. Canopy structure an...

    Xiong Xiong, Lejun Yu, Wanneng Yang, Meng Liu, Ni Jiang, Di Wu, Guoxing Chen, Lizhong Xiong, Kede Liu and Qian Liu

    Plant Methods 2017 13:7

    Published on: 31 January 2017

  15. Methodology

    Spatially-localized bench-top X-ray scattering reveals tissue-specific microfibril orientation in Moso bamboo

    Biological materials have a complex, hierarchical structure, with vital structural features present at all size scales, from the nanoscale to the macroscale. A method that can connect information at multiple l...

    Patrik Ahvenainen, Patrick G. Dixon, Aki Kallonen, Heikki Suhonen, Lorna J. Gibson and Kirsi Svedström

    Plant Methods 2017 13:5

    Published on: 9 January 2017

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