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Fig. 5 | Plant Methods

Fig. 5

From: New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i

Fig. 5

Cas9 transcripts and protein accumulation in stable transformed grapevine plantlets expressing each construction or in wild-type plantlets. (a) Cas9 transcripts levels from leaves collected on one-month grapevine plantlets. Asterisks indicate a statistically significant difference between two constructions highlighted by a bar (Wilcoxon test; ***, P < 0,001; N.S., no significant). (b) Immunodetection of Cas9 accumulation by western blotting in grapevine plantlets upon the expression of different genes and promoters. Asterisks indicate a statistically significant difference between two constructions highlighted by a bar (Wilcoxon test; *, P < 0,05; N.S., no significant). For both experiments, “n” represents the number of biological replicates. Each independent biological replicate involved sampling three leaves from three distinct plantlets. Different plantlets were sampled for each independent biological replicate. All the plantlets sampled correspond to those sequenced in Fig. 4

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Plant Methods

ISSN: 1746-4811