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Fig. 3 | Plant Methods

Fig. 3

From: New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i

Fig. 3

DsRed2 fluorescence emitted by transformed grapevine embryogenic cells, embryo and plantlets for transgenic lines containing each construction or wild-type. Embryogenic cells, embryo and plantlets growing on agar plate containing adapted media, were observed 3, 5 and 8 months after transformation, respectively. Brightfield (BF) and fluorescent (DsRed2) pictures of each stage are showed. (a) Non-transformed Wild-Type cv. ‘Chardonnay’ as a control of autofluorescence. (b) Wild-Type transformed with construct 1 (p35S::hCas9). (c) Wild-Type transformed with construct 2 (p35S::zCas9i). (d) Wild-Type transformed with construct 3 (pRPS5a::zCas9i)

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Plant Methods

ISSN: 1746-4811