Fig. 2From: New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9iDetailed process to obtain transformed grapevine embryogenic cells (cv. Chardonnay) and regenerated plantlets. Antibiotics selection for transformed cells allows to discern resistant white embryogenic cells (c, red outlined) and non-resistant black embryogenic cells (c, green outlined). (a-h) Indicate the different steps, directly described in this figure, of the plant method developped to obtain high frequency biallelic homozygous knock-out grapevine plantlets. BF: Brightfield. Figure created with biorender.comBack to article page