Skip to main content
Fig. 6 | Plant Methods

Fig. 6

From: New improvements in grapevine genome editing: high efficiency biallelic homozygous knock-out from regenerated plantlets by using an optimized zCas9i

Fig. 6

Off-target analysis in transgenic lines expressing construct 2. (a) Listing of predicted off-targets for both sgRNAs. One off-target is predicted for the sgRNA1 and sgRNA2 and 3 off-targets are predicted for sgRNA3. Sequence of different off-targets, the off-targets score risk and locus description are detailed in the second, third and fourth column respectively. “*” represent mismatch between sgRNA and off-target sequences. (b) Off-target 1 and 3 analysis on transgenic lines expressing construct 2. Off-target 1 and 3 have the most elevated score risk. Each off-target region has been amplified by PCR and sequenced. Sequencing results for each plantlet are presented in the third column

Back to article page

Plant Methods

ISSN: 1746-4811