Fig. 6
AraDQ-based image analysis of Pseudomonas-treated Arabidopsis seedlings having different genetic backgrounds. Seedlings of four different A. thaliana lines (Col-0, fls2, efr, and fls2/efr/cerk1 [fec]) were flood-inoculated with suspensions containing 7.5 × 105 colony-forming units (CFUs)/mL of wild-type (WT) and cmaA mutant strains of P. syringae pv. tomato DC3000, and their phenotypic responses were analyzed using AraDQ at 3 days post-inoculation (DPI). A Cropped images of flood-inoculated seedlings after normalization. B Fold changes in values of leaf area (cm2). The fold change values represent the means calculated using data obtained from six different seedlings. C Fold changes in values of green chromatic coordinate (GCC). The tables below each graph show that the relative fold change values for bacterial and mock treatments were estimated using the mean fold change values. Statistical significance was determined by one-way analysis of variance (ANOVA) and Tukey’s honestly significant difference (HSD) tests. Mean values marked with the same letter do not differ significantly (p < 0.05). Error bars indicate standard deviation. The data shown are representative of three independent experiments