Fig. 5
AraDQ-based image analysis of Arabidopsis seedlings treated with different concentrations of Pseudomonas strains. Seedlings of A. thaliana (Col-0) were flood-inoculated with six different concentrations of wild-type (WT) and cmaA and hrpA mutant strains of P. syringae pv. tomato DC3000, and the disease symptoms were analyzed using AraDQ at 3 days post-inoculation (DPI). A Visual comparison of flood-inoculated seedlings after normalization. B Fold changes in values of green chromatic coordinate (GCC). C Fold changes in values of leaf area (cm2). The fold change values represent the means calculated using data obtained from six different seedlings, and error bars indicate standard deviation. Statistical significance was determined by one-way analysis of variance (ANOVA) and Tukey’s honestly significant difference (HSD) tests. Mean values marked with the same letter do not differ significantly (p < 0.05). The data shown are representative of three independent experiments