Table 5 Ranking order of the genes as determined by the outcomes from the three tools
Treatments | Rank EEF-1α | Rank GAPDH | Rank β-tubulin | ||||||
|---|---|---|---|---|---|---|---|---|---|
BestKeeper | GeNorm | NormFinder | BestKeeper | GeNorm | NormFinder | BestKeeper | GeNorm | NormFinde | |
Control | 1 | 1 | 1 | 2 | 2 | 3 | 3 | 3 | 2 |
Melatonin + Cold stress | 1 | 1 | 2 | 3 | 4 | 3 | 2 | 3 | 1 |
Melatonin + High-temperature stress | 1 | 1 | 1 | 3 | 4 | 2 | 2 | 3 | 3 |
Melatonin + Salinity stress | 1 | 2 | 1 | 4 | 3 | 2 | 2 | 4 | 3 |
EBR + Cold stress | 1 | 1 | 2 | 2 | 4 | 1 | 3 | 3 | 3 |
EBR + High-temperature stress | 1 | 1 | 1 | 4 | 4 | 2 | 2 | 3 | 3 |
EBR + Salinity stress | 1 | 1 | 3 | 2 | 2 | 4 | 4 | 6 | 1 |
TiO2 NPs + Cold stress | 1 | 1 | 1 | 3 | 6 | 3 | 2 | 2 | 2 |
TiO2NPs + High-temperature | 1 | 1 | 1 | 4 | 6 | 3 | 2 | 3 | 2 |
TiO2 NPs + Salinity stress | 1 | 1 | 2 | 3 | 6 | 3 | 2 | 3 | 1 |
Cold plasma + Cold stress | 1 | 3 | 2 | 2 | 1 | 1 | 3 | 2 | 4 |
Cold plasma + High-temperature stress | 1 | 2 | 2 | 3 | 4 | 4 | 2 | 1 | 1 |
Cold plasma + Salinity stress | 2 | 2 | 1 | 3 | 4 | 3 | 1 | 1 | 3 |
Treatments | BestKeeper | GeNorm | NormFinder | BestKeeper | GeNorm | NormFinder | BestKeeper | GeNorm | NormFinde |
|---|---|---|---|---|---|---|---|---|---|
Rank eIF4A | Rank UBE2D2 | Rank 18Â S rRNA | |||||||
Control | 4 | 4 | 8 | 5 | 5 | 10 | 6 | 7 | 6 |
Melatonin + Cold stress | 4 | 2 | 8 | 5 | 6 | 10 | 9 | 9 | 6 |
Melatonin + High-temperature stress | 4 | 2 | 4 | 5 | 8 | 6 | 8 | 9 | 10 |
Melatonin + Salinity stress | 3 | 1 | 4 | 5 | 6 | 10 | 6 | 8 | 5 |
EBR + Cold stress | 4 | 2 | 8 | 5 | 5 | 10 | 10 | 10 | 5 |
EBR + High-temperature stress | 3 | 2 | 4 | 6 | 7 | 8 | 9 | 9 | 10 |
EBR + Salinity stress | 3 | 3 | 2 | 8 | 9 | 9 | 7 | 10 | 6 |
TiO2 NPs + Cold stress | 5 | 9 | 5 | 4 | 3 | 7 | 10 | 10 | 8 |
TiO2 NPs + High-temperature | 5 | 10 | 5 | 3 | 2 | 4 | 8 | 9 | 8 |
TiO2 NPs + Salinity stress | 7 | 10 | 4 | 3 | 2 | 5 | 10 | 9 | 6 |
Cold plasma + Cold stress | 4 | 4 | 3 | 5 | 5 | 5 | 8 | 10 | 7 |
Cold plasma + High-temperature stress | 5 | 9 | 5 | 4 | 3 | 3 | 10 | 10 | 8 |
Cold plasma + Salinity stress | 4 | 3 | 2 | 5 | 5 | 7 | 8 | 10 | 6 |
Treatments | Rank Actin 11 | Rank HSP 70 | Rank 25Â S rRNA | ||||||
|---|---|---|---|---|---|---|---|---|---|
BestKeeper | GeNorm | NormFinder | BestKeeper | GeNorm | NormFinder | BestKeeper | GeNorm | NormFinde | |
Control | 7 | 8 | 4 | 8 | 6 | 7 | 9 | 10 | 9 |
Melatonin + Cold stress | 6 | 5 | 4 | 7 | 10 | 7 | 8 | 7 | 9 |
Melatonin + High-temperature stress | 6 | 5 | 9 | 7 | 6 | 8 | 10 | 7 | 7 |
Melatonin + Salinity stress | 8 | 9 | 6 | 7 | 10 | 9 | 10 | 7 | 7 |
EBR + Cold stress | 6 | 7 | 6 | 7 | 8 | 4 | 8 | 6 | 7 |
EBR + High-temperature stress | 7 | 8 | 6 | 5 | 6 | 5 | 8 | 5 | 7 |
EBR + Salinity stress | 7 | 8 | 7 | 5 | 5 | 5 | 9 | 7 | 8 |
TiO2 NPs + Cold stress | 8 | 4 | 4 | 6 | 5 | 9 | 7 | 8 | 10 |
TiO2 NPs + High-temperature | 6 | 4 | 10 | 7 | 5 | 7 | 10 | 8 | 9 |
TiO2 NPs + Salinity stress | 5 | 4 | 8 | 6 | 5 | 7 | 9 | 8 | 10 |
Cold plasma + Cold stress | 6 | 6 | 8 | 7 | 7 | 6 | 10 | 9 | 10 |
Cold plasma + High-temperature stress | 7 | 5 | 6 | 6 | 6 | 7 | 8 | 8 | 9 |
Cold plasma + Salinity stress | 7 | 6 | 5 | 6 | 7 | 8 | 9 | 9 | 10 |
Treatments | Rank UbcH10 | ||
|---|---|---|---|
BestKeeper | GeNorm | NormFinder | |
Control | 10 | 9 | 9 |
Melatonin + Cold stress | 10 | 8 | 5 |
Melatonin + High-temperature stress | 9 | 10 | 9 |
Melatonin + Salinity stress | 9 | 5 | 8 |
EBR + Cold stress | 9 | 9 | 9 |
EBR + High-temperature stress | 10 | 10 | 9 |
EBR + Salinity stress | 6 | 4 | 10 |
TiO2 NPs + Cold stress | 9 | 8 | 6 |
TiO2 NPs + High-temperature | 9 | 7 | 6 |
TiO2NPs + Salinity stress | 8 | 7 | 9 |
Cold plasma + Cold stress | 9 | 8 | 9 |
Cold plasma + High-temperature stress | 9 | 7 | 10 |
Cold plasma + Salinity stress | 10 | 8 | 9 |