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Table 4 Expression stability values of candidate reference genes calculated by NormFinder.

From: Characterizing reference genes for high-fidelity gene expression analysis under different abiotic stresses and elicitor treatments in fenugreek leaves

Treatment

Stability values

 

EEF-1α

UBE2D2

β-tubulin

Actin 11

HSP70

GAPDH

UbcH10

eIF4A

25 S rRNA

18 S rRNA

Control

0.433

1.369

0.817

2.300

2.555

0.859

2.613

1.700

2.694

1.607

Melatonin + Cold stress

0.512

1.991

0.352

0.959

1.439

0.938

1.229

1.560

1.856

1.406

Melatonin + High-temperature stress

0.565

1.120

0.825

2.116

2.083

0.817

1.017

0.950

2.070

2.451

Melatonin + Salinity stress

0.405

2.025

0.678

1.648

1.825

0.438

1.737

0.710

1.660

1.194

EBR + Cold stress

0.424

2.376

0.705

1.378

1.287

0.537

2.048

1.650

1.635

1.309

EBR + High-temperature stress

0.602

1.267

0.759

1.144

1.123

0.710

1.441

0.860

1.196

2.350

EBR + Salinity stress

0.593

1.618

0.416

1.328

1.076

1.015

2.331

0.540

1.552

1.183

TiO2 NPs + Cold stress

0.659

1.753

0.694

1.046

2.211

1.098

1.314

1.200

2.534

2.190

TiO2 NPs + High-temperature

0.445

1.007

0.578

2.595

1.673

0.933

1.384

1.250

1.874

1.804

TiO2 NPs + Salinity stress

0.546

1.154

0.488

1.513

1.357

0.644

1.890

0.990

2.391

1.232

Cold plasma + Cold stress

0.502

1.063

0.803

2.202

1.544

0.898

3.031

1.090

3.137

1.630

Cold plasma + High-temperature stress

0.715

1.097

0.840

1.871

2.352

0.793

3.000

1.450

2.980

2.800

Cold plasma + Salinity stress

0.606

2.206

0.625

1.082

2.609

0.672

2.783

0.450

3.183

1.370

Total samples

0.580

1.532

0.753

1.646

1.800

0.828

1.554

1.056

1.641

1.550

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Plant Methods

ISSN: 1746-4811