Characterizing reference genes for high-fidelity gene expression analysis under different abiotic stresses and elicitor treatments in fenugreek leaves

Table 3 Analysis of ten candidate reference genes based CV for 13 different experimental treatments

Treatment	CV [%]
	EEF-1α	UBE2D2	β-tubulin	Actin 11	HSP70	GAPDH	UbcH10	eIF4A	25 S rRNA	18 S rRNA
Control	0.80	1.28	1.10	3.14	2.89	1.08	4.80	1.45	4.54	1.46
Melatonin + Cold stress	0.77	1.47	0.53	0.37	1.81	1.07	1.45	1.37	1.88	2.02
Melatonin + High-temperature stress	0.89	0.84	1.04	1.91	2.32	1.22	2.91	0.97	3.01	2.59
Melatonin + Salinity stress	0.62	1.48	1.03	1.86	2.23	0.78	2.43	0.77	2.75	1.48
EBR + Cold stress	0.55	2.16	1.15	1.13	1.57	0.79	2.57	1.76	2.89	2.99
EBR + High-temperature stress	0.21	1.16	1.15	1.04	1.19	0.95	5.97	0.88	2.36	2.63
EBR + Salinity stress	0.89	1.31	0.66	1.08	0.51	1.28	2.47	0.31	1.91	1.46
TiO₂ NPs + Cold stress	0.37	1.41	1.17	1.73	2.47	1.53	4.86	1.02	1.90	2.53
TiO₂ NPs + High-temperature	0.39	0.68	1.21	0.88	1.97	1.30	3.89	1.15	4.11	2.26
TiO₂ NPs + Salinity stress	0.62	0.84	0.91	1.45	1.74	0.94	1.26	0.99	1.87	1.92
Cold plasma + Cold stress	0.76	0.51	0.71	2.41	1.88	0.37	1.51	0.71	2.76	2.16
Cold plasma + High-temperature stress	1.05	0.63	1.81	1.90	2.73	1.87	1.69	1.27	1.97	2.81
Cold plasma + Salinity stress	1.03	1.78	0.20	0.81	2.55	0.77	2.57	0.28	1.87	3.44

ISSN: 1746-4811