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Table 2 Analysis of ten candidate reference genes using the BestKeeper algorithm based on quantification cycle

From: Characterizing reference genes for high-fidelity gene expression analysis under different abiotic stresses and elicitor treatments in fenugreek leaves

Treatment

Cq

 

EEF-1α

UBE2D2

β-tubulin

Actin 11

HSP70

GAPDH

UbcH10

eIF4A

25 S rRNA

18 S rRNA

Control

14.67 ab

22.30 c

17.50 b

26.00 b

21.00 d

16.00 a

17.00

14.50 d

19.00 f

19.60 e

Melatonin + Cold stress

14.73 ab

24.92 b

17.75 b

21.92 de

22.52 c

16.15 a

22.57 bc

16.32 bc

23.92 bc

23.34 b

Melatonin + High-temperature stress

14.71 ab

24.35 b

17.46 b

23.57 cd

24.63 b

16.00 a

20.21 e

17.78 ab

21.78 de

23.42 b

Melatonin + Salinity stress

14.74 ab

24.91 b

17.77 b

24.18 c

24.44 b

15.74 a

24.32 b

15.98 c

22.28 d

22.06 c

EBR + Cold stress

14.75 ab

24.62 b

17.77 b

21.70 de

23.11 b

15.90 a

27.12 a

16.61 bc

23.67 c

17.10 f

EBR + High-temperature stress

14.89 ab

19.10 e

17.83 b

23.66 cd

23.92 b

16.00 a

20.49 d

16.65 bc

20.35 e

21.71 cd

EBR + Salinity stress

14.95 ab

27.94 a

17.86 b

22.67 d

18.11 e

16.06 a

21.24 cd

14.05 d

19.70 ef

22.51 c

TiO2 NPs + Cold stress

13.00 b

23.09 bc

17.37 b

22.32 d

23.78 b

14.00 b

15.97 f

15.98 c

22.24 d

22.40 c

TiO2 NPs + High-temperature

14.64 ab

21.9 cd

17.23 b

23.39 cd

23.36 b

15.99 a

21.09 d

18.43 a

15.91 g

23.25 b

TiO2 NPs + Salinity stress

14.93 ab

24.26 b

17.59 b

21.45 de

23.52 b

16.00 a

22.65 bc

16.53 bc

24.67 b

23.34 b

Cold plasma + Cold stress

14.85 ab

24.13 b

17.70 b

22.05 d

23.90 b

15.33 a

20.59

15.46 c

20.66 e

22.60 bc

Cold plasma + High-temperature stress

15.55 a

22.81 c

19.74 a

17.22 f

28.66 a

15.98 a

22.24 c

16.74 bc

18.91 f

22.15 c

Cold plasma + Salinity stress

15.48 a

22.91 c

17.43 b

28.51 a

23.02 bc

16.00 a

21.54 cd

15.94 c

27.29 a

27.82 a

Max [Cq]

15.55

27.94

19.74

28.51

28.66

16.00

27.12

18.43

27.29

27.82

Min [Cq]

13.00

19.1

17.46

17.22

18.11

14.00

15.97

14.05

15.91

17.10

Geo Mean [Cq]

14.4

23.5

17.60

22.86

23.38

16.07

21. 54

16.24

21.60

22.46

SD [± Cq]

0.68

2.08

1.10

2.33

2.00

0.90

2.70

1.13

2.86

2.11

CV [%Cq]

4.80

8.75

6.20

10.70

8.40

5.60

12.50

6.75

13.20

9.35

Coeff. of Corr. [r]

0.99

0.89

0.99

0.81

-0.19

0.97

0.90

0.96

0.95

0.95

p-value

0.001

0.001

0.001

0.34

0.05

0.001

0.001

0.001

0.005

0.004

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Plant Methods

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